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The CRISPRi-dCpf1 system uses inactivated Cpf1 (dCpf1), which does not generate DSBsthis avoids the lethal issue caused by the low homologous recombination efficiency of Clostridia and achieved 3- to 100-fold gene silencing efficiency in 27 Clostridium strains
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All patients were titrated up to a maximum tolerated dose of 9mg or 12mg including placebo patients
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