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Following drying, one ml of hexane and chloroform (1:1, v/v) was added to reconstitute, then treated with ten l of N, O-Bis (trimethylsilyl) trifluoroacetamide using trimethyl-chlorosilane as the derivatization agent
This idea was also confirmed by comparing the weight of transgenic mice with GH receptor inactivation, IGF-I knockout and the double inactivation[36]
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