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After washing, BG-SS-Lumi4-Tb labelled cells were treated with agonist for 30 minutes at 37C to induce GLP-1R internalisation, placed on ice to arrest further trafficking, and residual surface GLP-1R was de-labelled by cleavage of the lanthanide using the cell-impermeant reducing agent 2-mercaptoethane sulfonate (Mesna, 100 mM in TNE buffer, pH 8.6) for 5 minutes
That is incomplete
Behavioral and care management support are important factors
All studies to date on GLP-1 medications show nutritional consequences
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