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Class NNMT Inhibitor (Nicotinamide N-methyltransferase) Molecular Weight 193.2 Da (small molecule) Half-life Under investigation Purity 98% (HPLC) Form Powder Storage 20C, dry, protected from moisture Reconstitution DMSO or sterile water (partial solubility) Mechanism of action 5-Amino-1-methylquinolinium (5-Amino-1MQ) is a competitive inhibitor of NNMT an enzyme overexpressed in adipose tissue in obesity that converts nicotinamide to 1-methylnicotinamide while consuming SAM (S-adenosyl methionine)
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To normalize target gene expression levels, the relative expression of the gene in each sample versus a control in comparison to the -actin gene was calculated according to the 2-Ct method (41)
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