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The RNA-dependent RNA polymerase (NSP12) and helicase (NSP13) of the replication-transcription complex (RTC) are the most conserved proteins when considering the rate of change at each amino acid site (homologies or nucleic acid diversity) over the course of the SARS-CoV-2 pandemic or conservation in sequence across HCoVs and the coronavirus family [62]
D., Vitonis, A
In our earlier experiments, we found that such treatment did not induce any morphological changes in the endothelial cells and while the MTT test (Abcam, Cambridge, UK) was used, it did not reduce viability either
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