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In previously published protocols different types of derivatization reagents (CMQT or CMLT), different pH of the buffer used in the protocols (7.4) and shorter time of sample reduction and derivatization (3 min) were applied (Tables 3 and 4.) Changing the conditions of reduction and derivatization increases the efficiency of this process, which may give higher final concentrations of the analytes
Western blotting HCT116 cells were seeded in a six-well plate and treated with GSTO1 inhibitors for 24 h
Yes, glutathione can be combined with other hair growth treatments like minoxidil or biotin, but always consult your doctor to ensure compatibility
