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In Boyden chamber assays, TB4 stimulated keratinocyte migration 23-fold over medium alone within 45 hours with as little as 10 pg sufficient to produce a potent stimulatory response
Lancet 397 , 17361748 (2021)
Practical recommendation: 27-gauge, 5/8-inch needle with regular bevel covers the widest range of patients and produces the lowest combined error rate for leakage, injection time, and patient discomfort
If you arent completely confident in your ability or feel worried, a safe choice may be to schedule an appointment to have it administered by a physician in their clinic, or to have your B12 shot administered at a pharmacy
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