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Orthotopic model of lung cancer Stably transfected KP tumor cells expressing luciferase (KP-Luc) were injected into the tail vein of 6-week-old C57BL/6 mice, with each mouse receiving a 100 L PBS suspension containing 5 10 5 proliferating cells
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The paraffin sections were sequentially incubated in xylene solution A and xylene solution B for 19 min, followed by incubation in anhydrous ethanol solution A, anhydrous ethanol solution B, and 75% alcohol for 6 min at RT
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