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For apoptosis assay, cells were suspended in Annexin V binding buffer (BD Science, New Jersey, USA) containing Annexin V-FITC (MBL International, Woburn, MA, USA) and propidium iodide (BD Science) for 15 min in dark
Be careful with using around the eye area, or if you have sensitive skin
Hair loss related to GHRP-6 use is unlikely and would more typically be associated with other hormonal imbalances or genetic factors
Practical takeaway: Most human users report noticeable appetite suppression during the first 3-7 days of loading, consistent with the animal data showing rapid onset followed by tolerance
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