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The rationale for stacking is that different peptides operate through different mechanisms and do not typically compete or interfere with each other when used at standard doses
The peptide mixture was loaded onto a reverse phase trap column (Thermo Scientific Acclaim PepMap100, 100 m 2 cm, nano Viper C18) connected to the C18-reversed phase analytical column (Thermo Scientific Easy Column, 10 cm long, 75 m inner diameter, 3 m resin) in buffer A (0.1%formic acid) and separated with a linear gradient of buffer B (84% acetonitrile and 0.1% formic acid) at a flow rate of 300 nl/min controlled by IntelliFlow technology
doi: 10.1007/s11947-016-1724-z 165
Dibutyryl-cGMP, a synthetic cGMP derivative, reduces ionomycin-stimulated eryptosis [170]
