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Of note, the commercial Iscoves Modified Dulbeccos Medium (IMDM) and Serum Free Expansion Medium (SFEM), which were used as the basal medium for erythroblast cultures, contain a supraphysiological concentration of cystine at 292 M as compared to physiological plasma concentration of 4075 M cysteine in SCD patients, which decreased further to 3060 M during vaso-occlusive pain episodes 36

However, in DEM treated cells (figure 5B) nuclear CMFDA fluorescence is lower than in control cells and DEM attenuates and postpones the nuclear GSH peak that takes place during cell proliferation

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Research on this application is lacking