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the gel purified His6-AV-Ubiquitin-semapeptide (nucleotide sequence) encoding gene PCR product and pET 24 plasmid vector was restriction digested (double digestion) with Ndel (NEB) + Xhol (NEB) at 37 C
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*Correspondence: Chuan Sun, [email protected] This article was submitted to Clinical Diabetes, a section of the journal Frontiers in Endocrinology Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
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