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treated cells were incubated with JC-1 dye (5 g/mL) for 30 min at 37 C, followed by fluorescence detection using a fluorescence microscope, where the red/green fluorescence ratio was calculated to quantify mitochondrial polarization status, as a higher ratio indicates healthier mitochondrial function while a lower ratio suggests mitochondrial depolarization and dysfunction

Differentiation and characterization of excitatory and inhibitory synapses by cryo-electron tomography and correlative microscopy

There is no evidence that GLP-1-based therapies increase the risk of cancer recurrence, but limited data exist specifically addressing this question

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