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Consistency matters: Pick a repeatable window each night (same bedtime routine) to reduce day-to-day variability in sleep, nutrition timing, and training effects that can influence GH signaling
To measure the early IgM response, the same dose of TNP-Ficoll (Biosearch Technologies F-1300-100) was administered i.p., and blood was collected one day previous injection and 3, 5, and 7 days post injection
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