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Our article charts the discovery and development of the long-acting GLP-1 analogs liraglutide and, subsequently, semaglutide
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The livers were minced and further digested using Hanks balanced salt solution supplemented with 0.1% collagenase for 30 min at 37 C under agitation using the gentle MACS Octo Dissociator (Miltenyi Biotec)
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