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Histological analysis of liver, kidney, and cardiac tissue showed no structural damage or inflammatory markers at therapeutic doses
Then the pellets were stained for 20 min in 100 l assay buffer containing 5 g ml 1 propidium iodide (Sigma-Aldrich, P4170) and 3 l Annexin VFITC (BioLegend, 0640906)
By analyzing the metabolic and functional cargoes of macrophage and neutrophil exosomes, we can distinguish patients with hyper-inflammatory, hyper-thrombotic, or hyper-suppressive phenotypes, guiding the precise implementation of anti-inflammatory, anticoagulant, or immunomodulatory treatments
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