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Accessed 9/27/18
As intrahepatic cholangiopathy remains unresolved by surgical intervention, patients develop cholangitis (50%), portal hypertension (60%), and progressive fibrosis, leading to liver failure (50%) requiring liver transplantation with lifelong immunosuppressants
Interestingly, we found that succinate accumulated only in Gclc- deficient B cells and not in Gclc- deficient effector T cells nor Tregs 43,44
Samples (25 g protein / sample) were run over a 10% SDS-PAGE, transferred onto nitrocellulose membranes at 4 C, and membranes were blocked with blocking buffer (Tris-buffered saline / 0.5% Tween 20/0.5% dry milk) for 1 h at room temperature with constant agitation
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