Given that NACA was able to counteract the effects of the ACOX1 gain-of-function mutation, antioxidant treatment was considered for patient#1 during a severe disease flare
Western Blot analysis for phosphor-JNK1/2, -Actin as a lading control
Jackson MJPS, Bolaos J, Bruckdorfer R, Carlssen H, Elliot RM, Flier J, Griffiths HR, Heales S, Holst B, Lorusso M, Lund E, Moskaug JO, Moser U, Di Paola M, Polidori MC, Signorile A, Stahl W, Via-Ribes J, Astley SB: Antioxidants, reactive oxygen and nitrogen species, gene induction and mitochondrial function
Glutathione proved significant ROS reduction following the mitomycin C test, carried out according to the following protocol: after ROS induction in skin cells through the use of the genotoxic agent mitomycin C, keratinocytes cells are treated with glutathione and placebo to evaluate the intracellular ROS reduction