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Hydroquinone A gold-standard inhibitor, hydroquinone suppresses melanocyte activity and is highly effective for melasma and PIH

LC-FL analysis Samples derivatized with monobromobimane (mBB) (Life Technologies) were separated with a Waters Symmetry C18 (ID, 250 4.6 mm) column (Waters Corp., Milford, MA, USA) with mobile phase A(0.25% acetic acid in H 2 O, pH 3.9), B(0.25% acetic acid: methanol = 7:3) and C(0.25% acetic acid: methanol = 1:1) with a linear gradient from A:B = 65:35 to 2:8 in 8 min with a flow rate of 1.5 ml/min, and remained with 100% B for additional 10 min, and then changed to 100% C in the following 6 min with a flow rate of 1.0 ml/min

3) Recycling of iron ions by degrading intracellular ferritin proteins is also a way to increase the capacity of unstable iron pools in cells

The longer days also mean naturally getting a little less sleep, so having darker under-eyes is common during this time