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Glucagon receptor activity is hypothesised to increase energy expenditure and promote fat breakdown (lipolysis), which researchers believe may contribute to the substantial weight loss observed in early trials though these mechanisms continue to be investigated
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Following this, they were washed three times with PBST (1X PBS and 0.05% Tween-20), then incubated with mouse IgG FITC conjugated secondary antibody (Invitrogen)
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These have mostly been observed in rodents, which are known to have more C-cells than humansbut it also has been reported in humans with liraglutide
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