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Therefore, supplementing feed with higher concentration of GSH (35 mM) compared to the dosages used for previous in vitro studies (5 mM) 15 probably results in an excess quantity of protective extracellular GSH, most likely counterbalancing some potentially negative effects on gastric epithelial cell health by GGT-induced generation of pro-oxidative GSH degradation products 15
Lysed cells or tissues were centrifuged at 12,000 g for 10 min, and the supernatant was collected
PMID 16493586
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