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Additionally to the glycine biosensor, we have also designed and built constructs constitutively expressing the GcvA transcription factor, GcvR transcription factor and pepD using the BBa_J23100 promoter found on the iGEM registry of Basic Biological Parts
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Fluorescent staining for casepase3/7 was done using CellEvent TM Caspase-3/7 Green Detection Reagent Kit (Thermo Fisher Scientific)
This overabundance of free radicals is a major contributor to many diseases, including heart disease and cancer, and it also plays a significant role in age-related changes and illnesses
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