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Room temperature or warm water is generally easier on the stomach than ice-cold water, which can temporarily slow gastric motility further
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Founders (tm1a Pamflneo/flneo) were screened for the presence of the targeted allele and neo cassette, and bred with FLP-Deleter (B6.129S4-Gt(ROSA)26Sortm1(FLP1) Dym/RainJ) to remove the neo cassette (to obtain tm1c Pam fl/fl allele) (Additional file 2: Fig
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