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JA and JA-Ile quantification in leaf tissues Metabolite extraction and quantification of JA and JA-Ile in leaf tissues were performed according to previously described methods using AB Sciex 4500 QTRAP triple quadrupole mass spectrometer (AB SCIEX, MA, USA) equipped with an ACQUITY UPLC BEH C18 column (Waters, Eschborn, Germany) (50 2.1 mm, 1.7 m) 45

Pre-loading syringes can expose the peptide to more air and contaminants, potentially affecting its stability and efficacy over time

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