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Standard laboratory handling considerations include
Half-Life: The free peptide has a short half-life (approximately 1 to 2 hours), which is why implant technology was developed for a sustained therapeutic effect in approved applications
Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis
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