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However, the precise role of 15LO1 and its products in disease pathogenesis remains enigmatic
10.1056/NEJMoa2215024 46
Chief among them: no controlled human safety data exist for the co-formulated blend, ghrelin-receptor desensitization over time is poorly characterized, sustained IGF-I elevation is a theoretical concern that has not been evaluated for this product, and GH secretagogues can affect glucose and insulin sensitivity
Quantitative real-time PCR (qRT-PCR): RNA extraction and cDNA synthesis To estimate the relative expression of Bax, caspase-3, caspase-9, and Bcl-2, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using a 25 L reaction volume with the SinaSyber Blue HF-qPCR master mix (CinnaGen, Tehran, Iran) and a StepOne real-time PCR system (Applied Biosystems, USA)
