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RIPA lysis was performed with purified human cells and 25 g of protein was loaded on 10% BisTris NuPAGE gels with MOPS SDS running buffer (Life Technologies)
Pyroptosis is a recently identified programmed cellular death process that is triggered by the activation of the NLRP3 inflammasome and mediated by GSDMD
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This prevents beta-3 receptor desensitization while maintaining the appetite suppression and metabolic benefits of tirzepatide
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