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Biochemical measurements Peptide hormones were measured using in-house radioimmunoassays: total GLP-1 (the sum of 7-36NH 2 , 9-36NH 2 and potential mid-terminal cleaved fragments) was measured using a C-terminal specific antibody targeting amidated forms of GLP-1 (code no
The tirzepatide arrangements in GLP-1R and GIPR have an angular difference of 8.3
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