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The samples were analyzed both non-ultrasonicated (SiNPs-W and SiNPs-S) and ultrasonicated (SiNPs-Wu and SiNPs-Su) using a probe sonication (Sonics VCX 750, model CV334, Sonics & Materials, Newtown, CT, USA) with the following parameters: 20 kHz, 5 min, 80% amplitude, pulse 15 sec, and pause 30 sec (as in Section 2.4.2)
FDA-related reports and medical reviews describe BPC-157 as an unapproved peptide with limited human evidence, so human safety remains uncertain
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Johns Hopkins University
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