Media formulas were as follows: Liver Perfusion Medium: HBSS, no calcium, no magnesium, no phenol red (Life Technologies, Carlsbad, CA), 0.5 mM EDTA, 0.5 mM EGTA, 25 mM HEPES, and penicillin-streptomycin (10,000 U/mL)
This includes the following patient groups: - Those who follow a strictly vegan diet - Those over the age of 50 - Those who take certain medications that are known to inhibit the absorption of B12 - Those who have an autoimmune disorder - Those who have gastrointestinal disorders - Those who have issues with stomach ulcers, atrophic gastritis, and other similar health problems SYMPTOMS OF A B12 DEFICIENCY Whether you fall into any of the categories above or not, there are certain signs to be aware of that could mean that you are suffering from a vitamin B12 deficiency
Avoid Alcohol : Refrain from consuming alcohol for at least 24 hours
Psychiatry 58 , 347354 (2005)