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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark
Obesity in pregnant women also increases cardiovascular dysfunction in the adult offspring, as well as the mother
Generation of GM mice with the MLC enhancer incorporated in site 2 To extend our study from cell lines to a whole animal context, we generated genetically modified mice by injecting Cas9 mRNA, sgRNA targeting site 2 and the circular donor plasmid into mouse zygotes (Table 1)
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