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RINm5F cells were treated with indicated chemicals for 17 hr, and then the cells were trypsinized and washed cells twice with cold BioLegends cell staining buffer, and then the cells were resuspended in Annexin V binding buffer at a concentration of 1 10 7 cells/ml
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Not making lifestyle changes during treatment Using GLP-1 medications as the sole weight loss strategy without modifying diet, exercise, sleep, or stress management is like building a house without a foundation
However, persistent or severe symptoms warrant GP consultation, particularly if they interfere with nutrition, hydration, or quality of life
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