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In this experimental platform, the anti-gravity calf-muscle group (gastrocnemius (Gas), plantaris (Pla), and soleus (Sol) muscles) and shank muscles (tibialis anterior (TA) and extensor digitorum longus (EDL)) were collected from four experimental groups of 8-week-old C57BL/6J male mice: i) control (without any treatment), ii) 5-days suspension-disuse, iii) 3-days LASSS pre-administration (about 50 g in drinking water/g body weight/day) followed by 5-days suspension-disuse treatment without LASSS supplementation, and iv) 3-days GSSSG pre-administration followed by suspension-disuse treatment ( n = 3 mice per group)

Cell-retained crystal violet was dissolved in 100 L of crystal violet elution buffer (50% of ethanol and 0.1% acetic acid)

Figure 3 ThMP kinase, which produces ThDP through ThMP phosphorylation and is known in thiamine-synthesizing organisms, has not been identified in animals

Nogueiras, R., Nauck, M