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Total protein (3040 g) was loaded in 10% and 420% acrylamide gel (Bio-Rad, Hercules, CA, USA) and transferred onto a polyvinylidene fluoride (PVDF) membrane and incubated with first antibodies in T-TBS with 1% bovine serum albumin for 12 h at 4 C
Smart meal timing, portion control, and adequate hydration are crucial in managing potential side effects and supporting weight management goals
Additionally, IBS patients with constipation have been found to have lower natural GLP-1 levels , and lower GLP-1 correlated with more pain, suggesting a deficiency of GLP-1 signaling might be related to IBS symptoms
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