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2.6 Protein sample preparation for mass spectrometry Protein samples (10 g/sample) were reduced with 5 mM Tris(2-carboxyethyl) phosphine and alkylated with 10 mM 2-chloroacetamide at room temperature for 20 min
In contrast to the UGT1A subfamily, the members of the gene subfamily contain a different set of exons (Tukey & Strassburg, 2000)
USA 107 , 1469114696 (2010)
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