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Beyond 48 hours, potency loss becomes measurable
After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol
This therapy, often administered through testosterone pellets inserted under the skin, works over time to elevate testosterone levels, improving blood flow, energy, mood, libido, and sexual desire
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