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These often feature single-strain formulations (Akkermansia only) or generic multi-strain probiotics without metabolic-specific targeting

Those sets of qRT-PCR probes were as follows: tumor necrosis factor (Tnf, Mm00443260), interleukin 1 beta (Il1b, Mm00434228), interleukin 6 (Il6, Mm00446190), C-C motif chemokine ligand 2 (Ccl2, Mm00441242), stearoyl-CoA desaturase 1 (Scd1, Mm00772290), and fatty acid synthase (Fasn, Mm00662319)

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As BPC157 is a short peptide lacking commercially available antibodies, we synthesized biotin-labeled BPC157 and performed coimmunoprecipitation (co-IP) assays using biotin-specific antibodies