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Flow cytometry (FACSCantoII, BD Biosciences, San Jose, USA) was used to analyze at least 10,000 PBMC per sample, and their population was defined by size and granularity ( Supplementary Figures 1AC )

Effect of flow on targeting and penetration of angiopep-decorated nanoparticles in a microfluidic model blood-brain barrier

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The analysis was conducted following the manufacturers protocol, except for scaling down the initial amount of the LDH assay buffer (from 500 l to 200 l) and incubating the samples at 23 C instead of the instructed 37 C optimized for mammalian cells