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Cells were imaged by time-lapse microscopy using a Zeiss LSM-780 inverted confocal laser-scanning microscope with a 63x/1.4 oil immersion objective and a 488 nm excitation wavelength and emissions at 508 and 583 nm for 2 minutes to record a baseline, followed by agonist stimulation at 100 nM for 5 minutes and 2 minutes exposure to 10 M FSK + 100 M IBMX to generate a maximal response
The plan is to follow the patients for 5 years and 5 weeks to target the first 1225 MACE [12]
Potential use of glutathione as a treatment for Parkinsons disease
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