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Timing: Inject at a consistent time and rotate injection sites systematically

While many patients have had safe treatment using oral selenium as monotherapy at 100200 mcg per day and long-term intakes from food and supplements of approximately 800 mcg per day are rated as safe, it is important to exclude selenium as a contributing factor to type 2 hyperthyroidism due to ingestion of high dietary selenium levels, for example, from overuse of sports nutrition supplements

Moreover, we transfected 293T cells with Myc-labeled ubiquitin and Flag-labeled KEAP1 plasmids for further ubiquitination assay