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Louis, MO) for 5 min at room temperature, followed by 4% paraformaldehyde (PFA) fixation (Himedia, Mumbai, India) for 1 h at room temperature
[42] For example it is officially indicated and approved for diagnostic evaluation of pituitary function and also for treatment of delayed or inadequate growth in children
We then used the model to study different dosing strategies and found that modest differences result from different dosing strategies as long as they are started early enough (Figure12)
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