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Metformin: an old but still the best treatment for type 2 diabetes

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To generate cells compatible with the CRISPR-Cas9 system, lentivirus was generated by co-transfection of LentiCas9-Blast (Addgene #52962) 126 along with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260) into HEK 293T using Lipofectamine 2000 (Life Technologies)

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1000 mg of glutathione is normally used in medicine

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