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(2011) 6:e24293
In the detection of total glutathione, first glutathione reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH), and then GSH reacts with DTNB to produce yellow TNB, and the amount of total glutathione determines the amount of yellow TNB formation, and the amount of total glutathione can be obtained by detecting the absorbance of A 412
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Common symptoms of low glutathione levels include chronic fatigue, frequent colds or flu, allergic reactions, and weight management difficulties
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