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Inflammatory cytokines such as TNF and IFN, which are induced during infection and in IBD, have been shown to increase intestinal permeability in general, although single inflammatory models yielded different results [114] while probiotics and commensals can reverse such inflammatory dysfunctions in human intestinal epithelial cells, e.g
coli containing pET-28b(+) based plasmids (pANT13-16, pANT18, pANT20-21, pANT35, pANT95, pJK1-3, pJK7-9 or site saturation variants of pANT35) were inoculated from an overnight preculture at a ratio of 1:100 in 96-deep-well plates equipped with a CR1296 sandwich cover (EnzyScreen) in 0.5 ml TB medium containing 50 g ml 1 kanamycin sulfate per well and incubated at 37 C, 300 rpm using a 5 cm shaking diameter for 2.5 h
Neurochem Res 35:743748 Reuter SE, Evans AM (2012) Carnitine and acylcarnitines: pharmacokinetic, pharmacological and clinical aspects
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