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First, samples were processed by adding lysis buffer (1.5% SDS/100 mM Tris-Cl), homogenized, and centrifuged to obtain supernatants
Numerous conditions can alter the level of GSH synthesis via changes in glutamate-cysteine ligase activity and expression of the GCLC gene such as oxidative stress, antioxidant levels, hormones, cell proliferation, and diabetes mellitus
doi: 10.1016/j.jtemb.2023.127235 49 HussainSKhanMSheikhTMMMumtazMZChohanTAShamimSet al
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