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In a frequently cited 1999 rat wound study in the Journal of Investigative Dermatology, topical Thymosin Beta-4 was associated with a 42% increase in reepithelialization at four days and as much as 61% at seven days compared with saline controls, alongside increased angiogenesis

This is based on how well the patient responds and the severity of their deficiency

The immunoblots were incubated with primary antibody at 4 C overnight followed by incubation with IR Dye-coupled secondary antibody and visualization using the LI-COR Odyssey Fc imaging system