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Membranes were blocked with 5% non-fat milk and incubated with mouse anti-5mC (#61480, Active motif) or anti-5hmC (#39999, Active motif) followed by a horseradish peroxidase-conjugated antibody (Santa Cruz Biotechnology)

But it's anecdote pointing straight at something his own lab published nearly thirty years ago

Energy utilization for survival and fertilization parsimonious quiescent sperm turn extravagant on motility activation in rat1

It regulates the expression of tight junction proteins, which close the gaps between intestinal cells to prevent the leakage of toxic substances into the bloodstream and subsequent systemic inflammation