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Affinity pulldown assay The proteins and amounts indicated in the figures were incubated in a 15 l reaction buffer AP (25 mM Tris-HCl, pH 7.5, 0.5 mM EDTA, 10% glycerol, 0.01% Igepal CA-630, 0.5 mM DTT) with 100 mM KCl, 2 mM MgCl 2 , and 8 U TurboNuclease (Accelagen, Cat#NC0298896) at room temperature for 20 min
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Conclusion In conclusion, understanding the power of retinol and peptides, specifically GHK-Cu, is key to unlocking the full potential of your skincare routine
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