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Early production of rAAV vectors required the use of auxiliary helper viruses, such as Ad, along with the transient co-transfection of two plasmids: one carrying the vector plasmid encoding the transgene of interest (GOI) flanked by the ITRs, and the second plasmid containing the AAV Rep, Cap and AAP functions
What also doesn't help is the lack of education about how thse drugs work to the general public
You should avoid injecting into the exact same spot every time to prevent the buildup of fatty tissue lumps called lipohypertrophy
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