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In vitro reactive oxygen species (ROS) assay ARPE-19 cells were cultured as above then seeded at a concentration of 2.5 10 4 cells/well (200 L) in fresh culture media in 96-well black walled, clear bottom tissue culture treated plates for fluorescence detection

Direct interaction between nuclear IGF-1R and DNA is evidenced by both electrophoretic mobility shift assays [109] and chromatin immunoprecipitation experiments [106]

Our findings, therefore, could aid person-centered diagnosis and outcomes, and timely successful treatment(s) of depression in older adults